The successful candidate will be an integral member of an innovative lab that bridges the fields of neuroscience and ophthalmology. Research projects focus on understanding (1) the molecular pathways that contribute to retinal neurodegeneration in diabetes using both mouse models of diabetic retinopathy and retinal neurons in culture and (2) how retinal neurons and blood vessels interact using mouse models and novel culture systems. The Principal Investigator is a clinician-scientist looking to work together with a highly-motivated individual to learn and employ a variety of strategies for in vivo characterization of the retina in mice, isolation and culture of retinal neurons and vasculature, and phenotypic/metabolic analyses both in vivo and in vitro, to address the questions above with the goal of identifying new targets to treat patients with diabetic retinopathy and other blinding retinal diseases. In addition to his/her critical role in performing experiments and generating data, the Research Technologist will also manage laboratory housekeeping tasks such as maintenance and ordering of supplies and equipment.
Specific duties & responsibilities:
The Research Technologist will follow previously-developed protocols and operate standard laboratory equipment in a molecular biology research laboratory in order to help answer the research questions posed. Under the close supervision of the Principal Investigator, the Research Technologist will also have the opportunity to optimize laboratory protocols and design original experiments when applicable. This is a unique opportunity to interact very closely with the Principal Investigator one-on-one regarding execution and interpretation of protocols while also learning cutting-edge techniques from several large highly-productive laboratories at Johns Hopkins in the Departments of Ophthalmology, Neurology, and the Institute of Cell Engineering.
Specific day-to-day tasks include molecular biology techniques (e.g. Western blotting, PCR), tissue culture (including the maintenance and differentiation of stem cells), and immunostaining. Additional duties include general oversight of equipment, ordering of supplies, and breeding, genotyping, and maintenance of a mouse colony. Some weekend availability (to be compensated with overtime pay) is required for maintenance of stem cells utilized by the laboratory for in vitro neuronal studies.
The ideal candidate will possess a meticulous attention to detail, excellent communication skills, and an innovative spirit, with potential for increased independence with time and/or commensurate with prior experience.
Minimum qualifications (mandatory):
Bachelor’s degree in Biology, Biochemistry, Chemistry, Neuroscience, Genetics or related field.
Some previous experience working in a molecular biology (or equivalent) laboratory is preferred.
Prior experience with tissue culture and mouse handling is preferred but not mandatory.
Special knowledge, skills, and abilities:
Experience with Microsoft Word, Excel, and Google Docs preferred.
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14 - $19.44 (commensurate with experience) Employee group: Full Time Schedule: Monday-Friday/8:30-5:00pm/40 hours Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10003003-SOM Oph Retina Service Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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