The Research Technologist will work in a laboratory which focuses on the mechanisms through which gut microbiota can affect neurogenesis and neuronal differentiation in the enteric nervous system. Under the direct supervision of the faculty, the individual will be responsible for performing routine and complex tests in a research laboratory and contributing to general maintenance of the laboratory, equipment and supplies. The individual will be responsible for mouse colony management and genotyping. He/She will perform basic lab functions and will help with routine experimental work like tissue processing, cell culture work and ordering related agents. This individual will also aid in variety of both routine and complex research experiments including molecular biology and histology experiments, analyzing data and making informal presentations to others about his/her work.
Specific Duties & Responsibilities: This individual will participate in planning studies and evaluating/interpreting test results utilizing a basic and learned knowledge of research laboratory procedures and practices involving neurogastroenterology disorders. These lab-based duties will include:
Operate standard equipment, use standard procedures for specific laboratory tests and preparation techniques.
Perform mice maintenance and generating mouse strains (breeding and genotyping of mice).
Process tissue for qPCR and PCR, histology immunohistochemistry, and immunoblotting.
Responsible for laboratory maintenance, preparing solutions and media and ordering supplies.
Identify and report problems in completion of routine lab processes.
Careful record keeping of sample information and results to guarantee quality of experimentation.
Assists with troubleshooting of technical and experimental issues.
Contributes to selecting, developing, performing and evaluating research methods; operates appropriate equipment.
Uses sterile techniques to avoid contaminating lab experiments. Basic knowledge of and employ good tissue culture techniques.
Uses various software applications, such as spreadsheets, relational databases, electronic mail, World Wide Web, statistical packages, and graphics packages.
Uses good practices for data record keeping and analysis.
Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
Monitors inventory levels and proper storage of materials and supplies in accordance with established policies and procedures.
Keeps current knowledge of vendors and suppliers of materials specific to the laboratory requirements.
Minimum Qualifications (Mandatory):
Bachelor’s degree in biology, chemistry, or related field required.
Some related post –undergraduate work experience preferred. Familiarity with basic laboratory research techniques is desired.
Special Knowledge, Skills, and Abilities:
This work requires precision and attention to detail.
Individual should have strong communication and interpersonal skills.
Must have the ability to read and interpret direction, both oral and written, organize and prioritize work and follow through on assignments.
Must be able to work successfully as part of a team in a dynamic laboratory.
Any Specific Physical Requirements for the Job:
Sitting in a normal seated position in office setting.
Standing and/or walking for extended periods of time.
Lifting and/or assisting patients during evaluations within crowded clinical environment.
Reaching by extending hand(s) or arm(s) in any direction.
Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s), for example, using a keyboard.
Communication skills using the spoken word.
Ability to see within normal parameters.
Ability to hear within normal range.
Ability to move about.
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $14.14 - $19.44 per hour Employee group: Full Time Schedule: Mon-Friday 8-5 Exempt Status: Non-Exempt Location: 04-MD:School of Medicine Campus Department name: 10002808-SOM DOM Gastroenterology Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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